| 质粒名称: | pLgw EcoDam-V5-RFC1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 59209 |
| 存储实验室: | Bas van Steensel |
|
载体骨架基本信息 |
|
| 载体名称: | HIV-CS-CG |
| 载体出品公司: | PMID 9733856 |
| 完整质粒大小: | 10221 bp |
| 载体类型: | 哺乳细胞表达、Lentiviral ; DamID |
| 筛选标记: | Zeo marker is outside the LTRs and will not be packaged into virus. |
| 载体抗性: | Chloramphenicol and Ampicillin |
| 生长温度: | 37 ℃ |
| 宿主菌: | ccdB Survival |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 启动子: | Heat Shock Minimal Promoter |
| 标签/融合蛋白: |
Dam (N terminal on backbone)
V5 (N terminal on backbone)
|
| 克隆方法: | Gateway Cloning |
| 5’测序引物 | pCasper-hs (GCAACTACTGAAATCTGCCAAG) |
|
文献引用方法 |
|
| 材料和方法部分: |
pLgw EcoDam-V5-RFC1 was a gift from Bas van Steensel (Addgene plasmid # 59209 ; http://n2t.net/addgene:59209 ; RRID:Addgene_59209)
|
| 参考文献部分: | Human heterochromatin proteins form large domains containing KRAB-ZNF genes. Vogel MJ, Guelen L, de Wit E, Peric-Hupkes D, Loden M, Talhout W, Feenstra M, Abbas B, Classen AK, van Steensel B. Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12. 10.1101/gr.5391806 PubMed 17038565 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLgw EcoDam-V5-RFC1 10221 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Mammalian DamID lentiviral vector for fusing gene of interest with
Dam-V5 using Gateway cloning .
ACCESSION .
VERSION .
KEYWORDS pLgw EcoDam-V5-RFC1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10221)
AUTHORS Vogel MJ, Guelen L, de Wit E, Peric-Hupkes D, Loden M, Talhout W,
Feenstra M, Abbas B, Classen AK, van Steensel B
TITLE Human heterochromatin proteins form large domains containing
KRAB-ZNF genes.
JOURNAL Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12.
PUBMED 17038565
REFERENCE 2 (bases 1 to 10221)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10221
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 616..814
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 832..1012
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1056..1181
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1674..1907
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2092..2136
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
promoter 2598..2836
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
primer_bind 2783..2804
/label=pCasper-hs
/note="Drosophila Hsp70 promoter, forward primer"
CDS 3731..3772
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
protein_bind 3788..3912
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 3937..3967
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 4021..4701
/codon_start=1
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
DPAY"
primer_bind complement(4087..4106)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 5021..5326
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 5205..5224
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(5367..5491)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
LTR 6022..6202
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6228..6245)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6234..6458
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6504..6932
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6591..6610)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6801..6822
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6941..6961)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6946..7276
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7127..7262
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7189..7208
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 7324..7371
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 7390..7764
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 7894..8015
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7931..7950)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7985..8004
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8064..8080)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8064..8080)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8077..8099)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8088..8104
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8112..8142)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8157..8178
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8295..8312)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8466..9054)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8546..8565)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9225..10085)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9848..9867
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10086..10190)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
1501 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
1861 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
2281 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcccccgg gctgcaggaa
2401 ttcagatctc ggccgcatat taagtgcatt gttctcgata ccgctaagtg cattgttctc
2461 gttagctcga tggacaagtg cattgttctc ttgctgaaag ctcgatggac aagtgcattg
2521 ttctcttgct gaaagctcga tggacaagtg cattgttctc ttgctgaaag ctcagtaccc
2581 gggagtaccc tcgaccgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc
2641 aattcaaaca agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca
2701 gctgaacaag ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa
2761 ccagcaacca agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa ttattgaata
2821 caagaagaga actctgaata ctttcaacaa gttaccgaga aagaagaact cacacacagc
2881 tagcgccacc atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct
2941 gcttgatgat attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg
3001 tgccgggtcg gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag
3061 cgacctgatc agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc
3121 acgcgagctg tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga
3181 gttcaacaaa agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta
3241 cggttacaac ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgttcggccg
3301 ctacaaaaaa ccctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa
3361 tgcctttttc tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt
3421 cgtctattgc gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca
3481 cacaaacagt tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt
3541 tgagcgccat attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta
3601 tcagcgcgca aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac
3661 acgtaaaaag gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa
3721 aaaaaccggt ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgatcgattt
3781 cgatcaccca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
3841 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
3901 ccagtcctat gggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg
3961 tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa
4021 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
4081 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
4141 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
4201 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt
4261 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
4321 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
4381 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
4441 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
4501 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
4561 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
4621 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggc ggggcgtaat
4681 ctagaggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
4741 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
4801 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
4861 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
4921 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
4981 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
5041 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
5101 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtccc
5161 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
5221 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
5281 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
5341 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
5401 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
5461 tttctcgttc agctttcttg tacaaagtgg ttcgatcgaa ccgcggtcta gactcgagac
5521 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgcttgtgcc
5581 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
5641 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
5701 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
5761 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
5821 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
5881 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
5941 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
6001 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
6061 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
6121 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
6181 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
6241 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
6301 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
6361 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
6421 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
6481 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
6541 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
6601 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
6661 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
6721 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
6781 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
6841 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa
6901 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
6961 tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta
7021 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
7081 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
7141 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
7201 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
7261 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
7321 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
7381 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
7441 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
7501 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
7561 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
7621 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
7681 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
7741 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
7801 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
7861 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
7921 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
7981 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
8041 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
8101 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
8161 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
8221 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
8281 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
8341 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
8401 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
8461 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
8521 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
8581 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
8641 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
8701 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
8761 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
8821 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
8881 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
8941 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
9001 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
9061 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
9121 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
9181 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
9241 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
9301 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
9361 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
9421 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
9481 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
9541 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
9601 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
9661 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
9721 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
9781 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
9841 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
9901 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
9961 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
10021 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
10081 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
10141 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
10201 cgaaaagtgc cacctgacgt c
//
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